Publication detail
Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions
Vaclav Diopan, Violetta Shestivska, Ondrej Zitka, Michaela Galiova, Vojtech Adam, Jozef Kaiser, Ales Horna, Karel Novotny, Miroslav Liska, Ladislav Havel, Josef Zehnalek, Rene Kizek
Czech title
Určení rostlinných thiolů pomocí chromatografie a kolorimetrie v listech salátu
English title
Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions
Type
journal article - other
Language
en
Original abstract
The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.
Czech abstract
Článek pojednává o možnostech určení rostlinných thiolů pomocí chromatografie a kolorimetrie v listech salátu dopovaných olovem. Kromě chromatografie a kolorimetrie je diskutován i možnost použití spektrometrie laserem indukovaného mikroplazmatu (LIBS) na mapovaní rozložení olova v kontaminovaních rostlinách.
English abstract
The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.
Keywords in English
Thiols, Liquid chromatography, Coulometric detection, Amperometric detection, Heavy metal, Laser induced breakdown spectroscopy
RIV year
2010
Released
12.01.2010
ISSN
1040-0397
Journal
ELECTROANALYSIS
Volume
22
Number
11
Pages from–to
1248–1259
Pages count
12
BIBTEX
@article{BUT49960,
author="Jozef {Kaiser} and Miroslav {Liška} and Michaela {Vašinová Galiová},
title="Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions",
journal="ELECTROANALYSIS",
year="2010",
volume="22",
number="11",
month="January",
pages="1248--1259",
issn="1040-0397"
}